Fig. 1

Standardization of qRT-PCR for determination of miR-122 levels. Circulating miR-122 levels were calculated using a standard curve obtained with synthetic miR-122 oligonucleotide. Synthetic miR-122 was 10-fold serially diluted and qRT-PCR was performed using TaqMan qRT-PCR assays. Standard curve for miR-122 generated is shown in (a) and the corresponding amplification plot is shown in (b)