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Fig. 2 | ExRNA

Fig. 2

From: Prediction and experimental confirmation of banana bract mosaic virus encoding miRNAs and their targets

Fig. 2

Experimental validation. a. RNA isolation from the banana plant. Lane 1- Before DNAse treatment. Lane 2- After DNAse treatment. b. Amplification of monocot actin gene. Analysed reaction products using agarose gel electrophoresis (1%) in 1× TAE. M - 1 Kb ladder. A1 - Monocot actin amplification from uninfected banana. A2 - Monocot actin amplification from BBrMV infected banana. NTC - Non-template control. c. Amplification of predicted vmiRNAs from BBrMV infected banana leaf sample. Analysed reaction products using agarose gel electrophoresis (4%) in 1× TAE. M - Low range DNA ladder. B1 - BBrMV miRNA 1. B2 - BBrMV miRNA 2. B3 - BBrMV miRNA 3. B4 - BBrMV miRNA 4. B5 - BBrMV miRNA 7. NTC - Non-template control. d. Amplification of BBrMV miRNA2 from BBrMV infected and uninfected banana samples. Analysed reaction products using agarose gel electrophoresis (4%) in 1× TAE. M - Low range marker. I - BBrMV infected banana plant showing the amplification of BBrMV miRNA2. Con - Uninfected banana plant showing the absence of BBrMV miRNA2 amplification. NTC - Non-template control

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