Fig. 2
From: Systemic spreading of exogenous applied RNA biopesticides in the crop plant Hordeum vulgare
Stylectomy technique applied for the barley- Sitobion avenae system. a Sitobion avenae feeding on a barley leaf shortly before cutting the mouthparts (stylet) with a tungsten needle connected to a microcautery device. b Injection of antimycotic, antibiotic and RNAse-inhibitor using a microcapillary at the beginning of the exudation period after overlaying with silicone oil. c Sampling of sieve-tube exudate from a cut aphid stylet under silicone oil with a microcapillary after an exudation period of 24 h. d Stylectomy of ATTO 488-labeled Shp-dsRNAA488 in sprayed barley leaves. Detection of Shp-dsRNAA488 (green) in the phloem sap droplet using stylectomy. Aphids were placed on non-sprayed leaf parts and stylectomy was performed 48 h after spray treatment with Shp-dsRNAA488