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Fig. 3 | ExRNA

Fig. 3

From: The abundance of a transfer RNA-derived RNA fragment small RNA subpopulation is enriched in cauda spermatozoa

Fig. 3

Experimental validation of enriched tRNA-derived RNA fragment small RNA abundance in cauda spermatozoa. a to f The sRNA sequencing identified trend of enriched tRF5 abundance in cauda spermatozoa was experimentally validated via the use of the RT-qPCR approach for the tRF5 sRNAs, tRF5Ala (a), tRF5Glu (b), tRF5Gly (c), tRF5Leu (d), tRF5Lys (e) and tRF5Val (f). For RT-qPCR assessments (right panel) the error bars represent the standard error of the mean (SEM) of three biological replicates. Asterisks indicate significant difference (p < 0.05 = *; p < 0.01 = **; p < 0.001 = ***) compared to tRF5 sRNA abundance quantification in caput spermatozoa

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