Fig. 3
From: Systemic spreading of exogenous applied RNA biopesticides in the crop plant Hordeum vulgare
Profiling of Shp-dsRNA-derived siRNAs in the phloem sap from barley leaves. Phloem sap was sampled by stylectomy. Total RNAs were isolated using a single cell RNA purification kit (Norgen Biotek Corp.). sRNA reads between 19 and 30 nt from Shp-dsRNA-sprayed and mock-treated barley leaves were mapped to Shp-dsRNA precursor [1]. Read coverage varied from 0 to 162 as indicated. Sequencing data are gained from 5 pooled separate phloem sap isolations experiments with a total sample amount of 10 μl phloem sap from sprayed leaves and 10 μl phloem sap from non-sprayed leaves, respectively