Fig. 6

Gene-specific qPCR analysis of fungal CYP51A, CYP51B, and CYP51C transcripts at 6 dpi (corresponding to 9 d after spraying). The FgCYP51 gene silencing was measured via the 2^−ΔΔCt method in which the expression of the respective FgCYP51 gene was normalized against the reference genes EF1α (translation elongation-factor 1 α) and ß-tubulin, and this Δ-Ct value was then normalized against the Δ-Ct of the GFP sprayed control. The reduction in fungal FgCYP51 gene expression on CYP3-dsRNA-sprayed leaves as compared with GFP-dsRNA-sprayed controls was statistically significant (*P < 0.05, **P < 0.01, ***P < 0.001; Student’s t test)