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Fig. 4 | ExRNA

Fig. 4

From: The abundance of a transfer RNA-derived RNA fragment small RNA subpopulation is enriched in cauda spermatozoa

Fig. 4

Assessment of the abundance of the ANGIOGENIN endonuclease in caput, corpus and cauda spermatozoa. The abundance of the ANG endonuclease in spermatozoa sourced from the caput, corpus and cauda segments of the mouse epididymis was assessed via a standard immunoblotting approach. The specificity of the anti-ANG antibodies was confirmed via the inclusion of whole tissue lysates sampled from the mouse liver and anti-GAPDH (GLYCERALDEHYDE 3-PHOSPHATE DEHYDROGENASE) antibodies were used as the internal loading control. The red colored arrowhead indicates the immunoblot product of the expected size specific to the ANG endonuclease (~ 15 kDa). The ANG and GAPDH antibodies were commercially sourced from suppliers Abcam (antibody ID: ab10600) and Sigma-Aldrich (product number: G9545), respectively

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ExRNA

ISSN: 2398-0060

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